Mice were mixed C57/BL6 and 129 background. lzip is a free, command-line tool for the compression of data it employs the LempelZivMarkov chain algorithm (LZMA) with a user interface that is familiar to users of usual Unix compression tools, such as gzip and bzip2. The following numbers of mice were analyzed from each group: C FLOX: n = 6 C MKO: n = 7 CR FLOX: n = 6 CR MKO: n = 7. RNA was isolated from TA/EDL muscles for microarray analysis. PGC-1a Overexpression Strategies for Neuroprotection in Parkinsons disease. Control (FLOX) and PGC-1a skeletal muscle specific knock out (MKO) mice were placed on a control diet or a calorie restriction diet for 12 weeks. These results demonstrate that PGC-1a is a major regulator of the mitochondrial response to CR in skeletal muscle, but surprisingly show that neither PGC-1a nor mitochondrial biogenesis in skeletal muscle are required for the metabolic benefits of CR. In contrast, gene expression profiling and electron microscopy demonstrated that PGC-1a is required for the full CR-induced increases in mitochondrial gene expression and mitochondrial density in skeletal muscle. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. Surprisingly, MKO mice demonstrated a normal improvement in glucose homeostasis in response to CR, indicating that skeletal muscle PGC-1a is dispensable for the whole-body benefits of CR. The file in this repository named contains all 69 genes in the experiment and their correlations to the compounds in the experiment (the. 'PGC1a antibodies' comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. PGC-1a protects against neurodegenerative diseases. (superoxide dismutase 2 (Sod2) and PPAR coactivator 1- (Pgc1a). This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homeostasis, and the development of obesity. Download PDF (zip) Save to Kindle Save to Dropbox Save to Google Drive. PGC-1 increases energy metabolism and weight loss. To test this model for the first time, we examined the metabolic and mitochondrial response to CR in mice lacking skeletal muscle PGC-1a (MKO). PGC-1 makes new mitochondria and improves its function. Consequently, many of the mitochondrial and metabolic benefits of CR are attributed to increased PGC-1a activity. PGC-1a is a transcriptional coactivator that regulates mitochondrial function and is induced by CR. phosphotyrosine interaction, PH domain and leucine zip. CR improves mitochondrial energy production, fuel oxidation and reactive oxygen species scavenging in skeletal muscle and other tissues, and these processes are thought to be critical to the benefits of CR. increases PGC-1a expression followed by mitochondrial biogenesis and fatty acid oxidation. Calorie restriction (CR) is a dietary intervention that extends lifespan and healthspan in a variety of organisms.
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